@article {6918,
	title = {Live imaging of nervous system development and function using light-sheet microscopy.},
	journal = {Molecular Reproduction and Development},
	volume = {82},
	year = {2013},
	month = {2015 Jul},
	pages = {605-18},
	abstract = {<p>In vivo imaging applications typically require carefully balancing conflicting parameters. Often it is necessary to achieve high imaging speed, low photo-bleaching, and photo-toxicity, good three-dimensional resolution, high signal-to-noise ratio, and excellent physical coverage at the same time. Light-sheet microscopy provides good performance in all of these categories, and is thus emerging as a particularly powerful live imaging method for the life sciences. We see an outstanding potential for applying light-sheet microscopy to the study of development and function of the early nervous system in vertebrates and higher invertebrates. Here, we review state-of-the-art approaches to live imaging of early development, and show how the unique capabilities of light-sheet microscopy can further advance our understanding of the development and function of the nervous system. We discuss key considerations in the design of light-sheet microscopy experiments, including sample preparation and fluorescent marker strategies, and provide an outlook for future directions in the field.</p>
},
	issn = {1098-2795},
	doi = {10.1002/mrd.22258},
	author = {Lemon, William C and Keller, Philipp J}
}
