@article {6385,
	title = {Acousto-optic laser scanning for multi-site photo-stimulation of single neurons in vitro.},
	journal = {Journal of Neural Engineering},
	volume = {7},
	year = {2010},
	month = {2010 Aug},
	pages = {045002},
	abstract = {<p>To study the complex synaptic interactions underpinning dendritic information processing in single neurons, experimenters require methods to mimic presynaptic neurotransmitter release at multiple sites with no physiological damage. We show that laser scanning systems built around large-aperture acousto-optic deflectors and high numerical aperture objective lenses provide the sub-millisecond, sub-micron precision necessary to achieve physiological, exogenous synaptic stimulation. Our laser scanning systems can produce the sophisticated spatio-temporal patterns of synaptic input that are necessary to investigate single-neuron dendritic physiology.</p>
},
	keywords = {Acoustic Stimulation, Acoustics, Action Potentials, Animals, Cells, Confocal, Cultured, Equipment Design, Equipment Failure Analysis, Hippocampus, Light, Microscopy, Neurons, Photic Stimulation, Rats, Reproducibility of Results, Sensitivity and Specificity, Sprague-Dawley},
	issn = {1741-2552},
	doi = {10.1088/1741-2560/7/4/045002},
	author = {Losavio, Bradley E and Iyer, Vijay and Patel, Saumil and Saggau, Peter}
}
