@article {2501,
	title = {The nuclear receptor E75A has a novel pair-rule-like function in patterning the milkweed bug, Oncopeltus fasciatus.},
	journal = {Developmental Biology},
	volume = {334},
	year = {2009},
	month = {2009 Oct 1},
	pages = {300-10},
	abstract = {<p>Genetic studies of the fruit fly Drosophila have revealed a hierarchy of segmentation genes (maternal, gap, pair-rule and HOX) that subdivide the syncytial blastoderm into sequentially finer-scale coordinates. Within this hierarchy, the pair-rule genes translate gradients of information into periodic stripes of expression. How pair-rule genes function during the progressive mode of segmentation seen in short and intermediate-germ insects is an ongoing question. Here we report that the nuclear receptor Of{\textquoteright}E75A is expressed with double segment periodicity in the head and thorax. In the abdomen, Of{\textquoteright}E75A is expressed in a unique pattern during posterior elongation, and briefly resembles a sequence that is typical of pair-rule genes. Depletion of Of{\textquoteright}E75A mRNA caused loss of a subset of odd-numbered parasegments, as well as parasegment 6. Because these parasegments straddle segment boundaries, we observe fusions between adjacent segments. Finally, expression of Of{\textquoteright}E75A in the blastoderm requires even-skipped, which is a gap gene in Oncopeltus. These data show that the function of Of{\textquoteright}E75A during embryogenesis shares many properties with canonical pair-rule genes in other insects. They further suggest that parasegment specification may occur through irregular and episodic pair-rule-like activity.</p>
},
	keywords = {Animals, Blastoderm, Body Patterning, Cytoplasmic and Nuclear, Embryo, Hemiptera, Insect Proteins, Nonmammalian, Receptors, RNA Interference},
	issn = {1095-564X},
	doi = {10.1016/j.ydbio.2009.06.038},
	author = {Erezyilmaz, Deniz F and Kelstrup, Hans C and Riddiford, Lynn M}
}
