@article {2679,
	title = {Genomic analysis of Drosophila neuronal remodeling: a role for the RNA-binding protein Boule as a negative regulator of axon pruning.},
	journal = {The Journal of Neuroscience: The Official Journal of the Society for Neuroscience},
	volume = {28},
	year = {2008},
	month = {2008 Jun 11},
	pages = {6092-103},
	abstract = {<p>Drosophila mushroom body (MB) gamma neurons undergo axon pruning during metamorphosis through a process of localized degeneration of specific axon branches. Developmental axon degeneration is initiated by the steroid hormone ecdysone, acting through a nuclear receptor complex composed of USP (ultraspiracle) and EcRB1 (ecdysone receptor B1) to regulate gene expression in MB gamma neurons. To identify ecdysone-dependent gene expression changes in MB gamma neurons at the onset of axon pruning, we use laser capture microdissection to isolate wild-type and mutant MB neurons in which EcR (ecdysone receptor) activity is genetically blocked, and analyze expression changes by microarray. We identify several molecular pathways that are regulated in MB neurons by ecdysone. The most striking observation is the upregulation of genes involved in the UPS (ubiquitin-proteasome system), which is cell autonomously required for gamma neuron pruning. In addition, we characterize the function of Boule, an evolutionarily conserved RNA-binding protein previously implicated in spermatogenesis in flies and vertebrates. boule expression is downregulated by ecdysone in MB neurons at the onset of pruning, and forced expression of Boule in MB gamma neurons is sufficient to inhibit axon pruning. This activity is dependent on the RNA-binding domain of Boule and a conserved DAZ (deleted in azoospermia) domain implicated in interactions with other RNA-binding proteins. However, loss of Boule does not result in obvious defects in axon pruning or morphogenesis of MB neurons, suggesting that it acts redundantly with other ecdyonse-regulated genes. We propose a novel function for Boule in the CNS as a negative regulator of developmental axon pruning.</p>
},
	keywords = {Animals, Antigens, Axons, CD8, Developmental, Drosophila, Drosophila Proteins, Ecdysone, Gene Expression Regulation, Genetically Modified, Genomics, Green Fluorescent Proteins, Larva, Microarray Analysis, Mushroom Bodies, Mutation, Neurons, Receptors, RNA-Binding Proteins, Steroid},
	issn = {1529-2401},
	doi = {10.1523/JNEUROSCI.0677-08.2008},
	author = {Hoopfer, Eric D and Penton, Andrea and Watts, Ryan J and Luo, Liqun}
}
