@article {2503,
	title = {Insulin/IGF signaling regulates the change in commitment in imaginal discs and primordia by overriding the effect of juvenile hormone.},
	journal = {Developmental Biology},
	volume = {324},
	year = {2008},
	month = {2008 Dec 15},
	pages = {258-65},
	abstract = {<p>At the beginning of the final larval (fifth) instar of Manduca sexta, imaginal precursors including wing discs and eye primordia initiate metamorphic changes, such as pupal commitment, patterning and cell proliferation. Juvenile hormone (JH) prevents these changes in earlier instars and in starved final instar larvae, but nutrient intake overcomes this effect of JH in the latter. In this study, we show that a molecular marker of pupal commitment, broad, is up-regulated in the wing discs by feeding on sucrose or by bovine insulin or Manduca bombyxin in starved final instar larvae. This effect of insulin could not be prevented by JH. In vitro insulin had no effect on broad expression but relieved the suppression of broad expression by JH. This effect of insulin was directly on the disc as shown by its reduction in the presence of insulin receptor dsRNA. In starved penultimate fourth instar larvae, broad expression in the wing disc was not up-regulated by insulin. The discs became responsive to this action of insulin during the molt to the fifth instar together with the ability to become pupally committed in response to 20-hydroxyecdysone. Thus, the Manduca bombyxin acts as a metamorphosis-initiating factor in the imaginal precursors.</p>
},
	keywords = {Animals, Cells, Cloning, Cultured, Developmental, Ecdysterone, Gene Expression Regulation, Insect Proteins, Insulin, Juvenile Hormones, Larva, Manduca, Messenger, Molecular, Receptor, RNA, RNA Interference, Signal Transduction, Somatomedins, Sucrose, Wing},
	issn = {1095-564X},
	doi = {10.1016/j.ydbio.2008.09.017},
	author = {Koyama, Takashi and Syropyatova, Maria O and Riddiford, Lynn M}
}
