@article {2404,
	title = {IRES-mediated functional coupling of transcription and translation amplifies insulin receptor feedback.},
	journal = {Genes \& Development},
	volume = {21},
	year = {2007},
	month = {2007 Jan 15},
	pages = {175-83},
	abstract = {<p>It is generally accepted that the growth rate of an organism is modulated by the availability of nutrients. One common mechanism to control cellular growth is through the global down-regulation of cap-dependent translation by eIF4E-binding proteins (4E-BPs). Here, we report evidence for a novel mechanism that allows eukaryotes to coordinate and selectively couple transcription and translation of target genes in response to a nutrient and growth signaling cascade. The Drosophila insulin-like receptor (dINR) pathway incorporates 4E-BP resistant cellular internal ribosome entry site (IRES) containing mRNAs, to functionally couple transcriptional activation with differential translational control in a cell that is otherwise translationally repressed by 4E-BP. Although examples of cellular IRESs have been previously reported, their critical role mediating a key physiological response has not been well documented. Our studies reveal an integrated transcriptional and translational response mechanism specifically dependent on a cellular IRES that coordinates an essential physiological signal responsible for monitoring nutrient and cell growth conditions.</p>
},
	keywords = {5{\textquoteright} Untranslated Regions, Animals, Biological, Drosophila melanogaster, Drosophila Proteins, Eukaryotic Initiation Factor-4E, Feedback, Forkhead Transcription Factors, Gene Expression Regulation, Genetic, Insulin, Models, Phosphorylation, Physiological, Protein Biosynthesis, Receptor, Ribosomes, Signal Transduction, Transcription, Transfection},
	issn = {0890-9369},
	doi = {10.1073/pnas.1100640108},
	author = {Marr, Michael T and D{\textquoteright}Alessio, Joseph A and Puig, Oscar and Tjian, Robert}
}
