@article {2420,
	title = {Myc-interacting protein 1 target gene profile: a link to microtubules, extracellular signal-regulated kinase, and cell growth.},
	journal = {Proceedings of the National Academy of Sciences of the United States of America},
	volume = {101},
	year = {2004},
	month = {2004 Jan 13},
	pages = {458-63},
	abstract = {<p>To study the role of the transcription factor Myc-interacting protein 1 (MIZ-1) in activating various target genes after induction with the microtubule disrupting agent T113242, we have used small interfering RNA duplexes (siRNAs) to knockdown the expression of MIZ-1. As expected, depletion of MIZ-1 resulted in the inhibition of T113242-dependent activation of the low-density lipoprotein receptor (LDLR) gene in hepatocytes. Cells transfected with MIZ-1 siRNAs also exhibited growth arrest. In addition, inhibition of the extracellular signal-regulated kinase (ERK) pathway inhibited T113242-induced nuclear accumulation of MIZ-1 and activation of LDLR. Gene expression microarray analysis under various induction conditions identified other T113242-activated genes affected by a decrease in MIZ-1 and inhibition of the ERK pathway. We also found that the accumulation of MIZ-1 in the nucleus is influenced by cell-cell contact and/or growth. Taken together, our studies suggest that MIZ-1 regulates a specific set of genes that includes LDLR and that the ERK pathway plays a role in the activation of target promoters by MIZ-1.</p>
},
	keywords = {Cell Division, Cell Line, DNA-Binding Proteins, Flow Cytometry, Fluorescent Antibody Technique, Gene Expression Profiling, Humans, Indirect, Kruppel-Like Transcription Factors, Mitogen-Activated Protein Kinases, RNA Interference, Transcription Factors},
	issn = {0027-8424},
	doi = {10.1073/pnas.1100640108},
	author = {Ziegelbauer, Joseph and Wei, Joyce and Tjian, Robert}
}
