@article {2428,
	title = {Redundant role of tissue-selective TAF(II)105 in B lymphocytes.},
	journal = {Molecular and Cellular Biology},
	volume = {22},
	year = {2002},
	month = {2002 Sep},
	pages = {6564-72},
	abstract = {<p>Regulated gene expression is a complex process achieved through the function of multiple protein factors acting in concert at a given promoter. The transcription factor TFIID is a central component of the machinery regulating mRNA synthesis by RNA polymerase II. This large multiprotein complex is composed of the TATA box binding protein (TBP) and several TBP-associated factors (TAF(II)s). The recent discovery of multiple TBP-related factors and tissue-specific TAF(II)s suggests the existence of specialized TFIID complexes that likely play a critical role in regulating transcription in a gene- and tissue-specific manner. The tissue-selective factor TAF(II)105 was originally identified as a component of TFIID derived from a human B-cell line. In this report we demonstrate the specific induction of TAF(II)105 in cultured B cells in response to bacterial lipopolysaccharide (LPS). To examine the in vivo role of TAF(II)105, we have generated TAF(II)105-null mice by homologous recombination. Here we show that B-lymphocyte development is largely unaffected by the absence of TAF(II)105. TAF(II)105-null B cells can proliferate in response to LPS, produce relatively normal levels of resting antibodies, and can mount an immune response by producing antigen-specific antibodies in response to immunization. Taken together, we conclude that the function of TAF(II)105 in B cells is likely redundant with the function of other TAF(II)105-related cellular proteins.</p>
},
	keywords = {Animals, B-Lymphocytes, Blotting, Cell Division, Cell Separation, DNA-Binding Proteins, Dose-Response Relationship, Drug, Erythrocytes, Flow Cytometry, Genetic, Immunoglobulin A, Immunoglobulin G, Immunoglobulin M, Lipopolysaccharides, Messenger, Mice, Precipitin Tests, RNA, Spleen, TATA-Binding Protein Associated Factors, Time Factors, Transcription, Transcription Factor TFIID, Transcription Factors, Transcriptional Activation, Western},
	issn = {0270-7306},
	doi = {10.1073/pnas.1100640108},
	author = {Freiman, Richard N and Albright, Shane R and Chu, Leslie E and Zheng, Shuang and Liang, Hong-Erh and Sha, William C and Tjian, Robert}
}
