@article {1183,
	title = {Precise identification of individual promoters for transcription of each strand of human mitochondrial DNA.},
	journal = {Cell},
	volume = {36},
	year = {1984},
	month = {1984 Mar},
	pages = {635-43},
	abstract = {<p>The major site of in vivo transcriptional initiation for both heavy and light strands of human mitochondrial DNA is the displacement-loop region. Transcripts synthesized in vitro by human mitochondrial RNA polymerase were mapped to the nucleotide level and have identical 5{\textquoteright} end map positions to those reported for in vivo primary transcripts. An ordered series of deletion clones, whose template sequences were truncated at either the 5{\textquoteright} or 3{\textquoteright} end, was used to identify the precise mitochondrial DNA sequence required for initiation of transcription. The data provide a definitive assignment of the promoter for heavy-strand transcription occurring within -16 to +7 of the transcriptional start site 16 nucleotides upstream of the 5{\textquoteright} end of the gene for tRNAPhe and of the promoter for light-strand transcription occurring within -28 to +16 of the transcriptional start site at the 5{\textquoteright} end of "7S RNA." Within each control sequence is a candidate promoter whose consensus sequence is 5{\textquoteright}-CANACC(G)CC(A)AAAGAPyA-3{\textquoteright} and in both cases transcriptional initiation occurs within six to eight nucleotides of the 3{\textquoteright} end of this sequence. The transcriptional start site is an integral part of each promoter and each promoter can function in the absence of the other.</p>
},
	keywords = {Chromosome Deletion, Chromosome Mapping, DNA, DNA-Directed RNA Polymerases, Genes, Genetic, Humans, Mitochondrial, Operon, Regulator, RNA, Transcription, Transfer},
	issn = {0092-8674},
	doi = {10.1101/gad.1352105},
	author = {Chang, D D and Clayton, D A}
}
