@article {46325,
	title = {Ultrastructurally smooth thick partitioning and volume stitching for large-scale connectomics.},
	journal = {Nature Methods},
	volume = {12},
	year = {2015},
	month = {2015 Feb 16},
	pages = {319-22},
	abstract = {<p>Focused-ion-beam scanning electron microscopy (FIB-SEM) has become an essential tool for studying neural tissue at resolutions below 10 nm {\texttimes} 10 nm {\texttimes} 10 nm, producing data sets optimized for automatic connectome tracing. We present a technical advance, ultrathick sectioning, which reliably subdivides embedded tissue samples into chunks (20 μm thick) optimally sized and mounted for efficient, parallel FIB-SEM imaging. These chunks are imaged separately and then {\textquoteright}volume stitched{\textquoteright} back together, producing a final three-dimensional data set suitable for connectome tracing.</p>
},
	issn = {1548-7105},
	doi = {10.1038/nmeth.3292},
	author = {Hayworth, Kenneth J and Xu, C Shan and Lu, Zhiyuan and Knott, Graham W and Fetter, Richard D and Tapia, Juan Carlos and Lichtman, Jeff W and Hess, Harald F}
}
