@article {46368,
	title = {Labeling of active neural circuits in vivo with designed calcium integrators.},
	journal = {Science},
	volume = {347},
	year = {2015},
	month = {2015 Feb 13},
	pages = {755-60},
	abstract = {<p>The identification of active neurons and circuits in vivo is a fundamental challenge in understanding the neural basis of behavior. Genetically encoded calcium (Ca(2+)) indicators (GECIs) enable quantitative monitoring of cellular-resolution activity during behavior. However, such indicators require online monitoring within a limited field of view. Alternatively, post hoc staining of immediate early genes (IEGs) indicates highly active cells within the entire brain, albeit with poor temporal resolution. We designed a fluorescent sensor, CaMPARI, that combines the genetic targetability and quantitative link to neural activity of GECIs with the permanent, large-scale labeling of IEGs, allowing a temporally precise "activity snapshot" of a large tissue volume. CaMPARI undergoes efficient and irreversible green-to-red conversion only when elevated intracellular Ca(2+) and experimenter-controlled illumination coincide. We demonstrate the utility of CaMPARI in freely moving larvae of zebrafish and flies, and in head-fixed mice and adult flies.</p>
},
	issn = {1095-9203},
	doi = {10.1126/science.1260922},
	author = {Fosque, Benjamin F and Sun, Yi and Dana, Hod and Yang, Chao-Tsung and Ohyama, Tomoko and Tadross, Michael R and Patel, Ronak and Zlatic, Marta and Kim, Douglas S and Ahrens, Misha B and Jayaraman, Vivek and Looger, Loren L and Schreiter, Eric R}
}
