@article {46706,
	title = {Background free imaging of single mRNAs in live cells using split fluorescent proteins.},
	journal = {Scientific Reports},
	volume = {4},
	year = {2014},
	month = {2014 Jan 9},
	pages = {3615},
	abstract = {<p>We describe a technique for imaging single mRNAs in living cells based on fluorescent protein (FP) complementation. We employ the high affinity interaction between the bacterial phage MS2/PP7 coat proteins and their respective RNA binding motifs as an RNA scaffold to bring two halves of a split-FP together to image single reporter mRNAs without background fluorescence.</p>
},
	keywords = {Bacteriophages, Fluorescence, Luminescent Proteins, RNA, Messenger},
	issn = {2045-2322},
	doi = {10.1038/srep03615},
	author = {Wu, Bin and Chen, Jiahao and Singer, Robert H}
}
