@article {47262,
	title = {Simultaneous denoising, deconvolution, and demixing of calcium imaging data.},
	journal = {Neuron},
	volume = {89},
	year = {2016},
	month = {2016 Jan 20},
	pages = {285-99},
	abstract = {<p>We present a modular approach for analyzing calcium imaging recordings of large neuronal ensembles. Our goal is to simultaneously identify the locations of the neurons, demix spatially overlapping components, and denoise and deconvolve the spiking activity from the slow dynamics of the calcium indicator. Our approach relies on a constrained nonnegative matrix factorization that expresses the spatiotemporal fluorescence activity as the product of a spatial matrix that encodes the spatial footprint of each neuron in the optical field and a temporal matrix that characterizes the calcium concentration of each neuron over time. This framework is combined with a novel constrained deconvolution approach that extracts estimates of neural activity from fluorescence traces, to create a spatiotemporal processing algorithm that requires minimal parameter tuning. We demonstrate the general applicability of our method by applying it to in\&nbsp;vitro and in\&nbsp;vivo multi-neuronal imaging data, whole-brain light-sheet imaging data, and dendritic imaging data.</p>
},
	issn = {1097-4199},
	doi = {10.1016/j.neuron.2015.11.037},
	author = {Pnevmatikakis, Eftychios A and Soudry, Daniel and Gao, Yuanjun and Machado, Timothy A and Merel, Josh and Pfau, David and Reardon, Thomas and Mu, Yu and Lacefield, Clay and Yang, Weijian and Ahrens, Misha and Bruno, Randy and Jessell, Thomas M and Peterka, Darcy S and Yuste, Rafael and Paninski, Liam}
}
