@article {47299,
	title = {Calcium imaging of neural circuits with extended depth-of-field light-sheet microscopy.},
	journal = {Optics Letters},
	volume = {41},
	year = {2016},
	month = {2016 Feb 17},
	pages = {855-8},
	abstract = {<p>Increasing the volumetric imaging speed of light-sheet microscopy will improve its ability to detect fast changes in neural activity. Here, a system is introduced for brain-wide imaging of neural activity in the larval zebrafish by coupling structured illumination with cubic phase extended depth-of-field (EDoF) pupil encoding. This microscope enables faster light-sheet imaging and facilitates arbitrary plane scanning{\textemdash}removing constraints on acquisition speed, alignment tolerances, and physical motion near the sample. The usefulness of this method is demonstrated by performing multi-plane calcium imaging in the fish brain with a\&nbsp;416{\texttimes}832{\texttimes}160  μm\&nbsp;field of view at 33\&nbsp;Hz. The optomotor response behavior of the zebrafish is monitored at high speeds, and time-locked correlations of neuronal activity are resolved across its brain.</p>
},
	doi = {10.1364/OL.41.000855},
	author = {Quirin, Sean and Vladimirov, Nikita and Yang, Chao-Tsung and Peterka, Darcy S and Yuste, Rafael and Ahrens, Misha B}
}
