@article {47307,
	title = {Transcriptomes of lineage-specific Drosophila neuroblasts profiled by genetic targeting and robotic sorting.},
	journal = {Development (Cambridge, England)},
	volume = {143},
	year = {2016},
	month = {2016 Feb 1},
	pages = {411-21},
	abstract = {<p>A brain consists of numerous distinct neurons arising from a limited number of progenitors, called neuroblasts in Drosophila. Each neuroblast produces a specific neuronal lineage. To unravel the transcriptional networks that underlie the development of distinct neuroblast lineages, we marked and isolated lineage-specific neuroblasts for RNA sequencing. We labeled particular neuroblasts throughout neurogenesis by activating a conditional neuroblast driver in specific lineages using various intersection strategies. The targeted neuroblasts were efficiently recovered using a custom-built device for robotic single-cell picking. Transcriptome analysis of mushroom body, antennal lobe and type II neuroblasts compared with non-selective neuroblasts, neurons and glia revealed a rich repertoire of transcription factors expressed among neuroblasts in diverse patterns. Besides transcription factors that are likely to be pan-neuroblast, many transcription factors exist that are selectively enriched or repressed in certain neuroblasts. The unique combinations of transcription factors present in different neuroblasts may govern the diverse lineage-specific neuron fates.</p>
},
	issn = {1477-9129},
	doi = {10.1242/dev.129163},
	author = {Yang, Ching-Po and Fu, Chi-Cheng and Sugino, Ken and Liu, Zhiyong and Ren, Qingzhong and Liu, Ling-Yu and Yao, Xiaohao and Lee, Luke P and Lee, Tzumin}
}
