@article {47547,
	title = {GFP-aequorin protein sensor for ex~vivo and in~vivo imaging of Ca(2+) dynamics in high-Ca(2+) organelles.},
	journal = {Cell Chemical Biology},
	year = {2016},
	month = {2016 Jun 1},
	abstract = {<p>Proper functioning of organelles such as the ER or the Golgi apparatus requires luminal accumulation of Ca(2+) at high concentrations. Here we describe a\&nbsp;ratiometric low-affinity Ca(2+) sensor of the GFP-aequorin protein (GAP) family optimized for measurements in high-Ca(2+) concentration environments. Transgenic animals expressing the ER-targeted sensor allowed monitoring of Ca(2+) signals inside the organelle. The use of the sensor was demonstrated under three experimental paradigms: (1) ER Ca(2+) oscillations in cultured astrocytes, (2) ex\&nbsp;vivo functional mapping of cholinergic receptors triggering ER Ca(2+) release in acute hippocampal slices from transgenic mice, and (3) in\&nbsp;vivo sarcoplasmic reticulum Ca(2+) dynamics in the muscle of transgenic flies. Our results provide proof of the suitability of the new biosensors to monitor Ca(2+) dynamics inside intracellular organelles under physiological conditions and open an avenue to explore complex Ca(2+) signaling in animal models of health and disease.</p>
},
	issn = {2451-9456},
	doi = {10.1016/j.chembiol.2016.05.010},
	author = {Navas-Navarro, Paloma and Rojo-Ruiz, Jonathan and Rodriguez-Prados, Macarena and Ganfornina, Mar{\'\i}a Dolores and Looger, Loren L and Alonso, Mar{\'\i}a Teresa and Garc{\'\i}a-Sancho, Javier}
}
