@article {47549,
	title = {Frealign: an exploratory tool for single-particle cryo-EM.},
	journal = {Methods in Enzymology},
	year = {2016},
	month = {2016 Jun 07},
	abstract = {<p>Frealign is a software tool designed to process electron microscope images of single molecules and complexes to obtain reconstructions at the highest possible resolution. It provides a number of refinement parameters and options that allow users to tune their refinement to achieve specific goals, such as masking to classify selected regions within a particle, control over the refinement of specific alignment parameters to accommodate various data collection schemes, refinement of pseudosymmetric particles, and generation of initial maps. This chapter provides a general overview of Frealign functions and a more detailed guide to using Frealign in typical scenarios.</p>
},
	keywords = {High resolution; Refinement; Classification; Contrast transfer function; Masking; Asymmetry},
	doi = {10.1016/bs.mie.2016.04.013},
	author = {Grigorieff, Nikolaus}
}
