@article {47885,
	title = {Automatic estimation and correction of anisotropic magnification distortion in electron microscopes.},
	journal = {Journal of Structural Biology},
	volume = {192},
	year = {2015},
	month = {2015 Nov},
	pages = {204-8},
	abstract = {<p>We demonstrate a significant anisotropic magnification distortion, found on an FEI Titan Krios microscope and affecting magnifications commonly used for data acquisition on a Gatan K2 Summit detector. We describe a program (mag_distortion_estimate) to automatically estimate anisotropic magnification distortion from a set of images of a standard gold shadowed diffraction grating. We also describe a program (mag_distortion_correct) to correct for the estimated distortion in collected images. We demonstrate that the distortion present on the Titan Krios microscope limits the resolution of a set of rotavirus VP6 images to \~{}7 {\r A}, which increases to \~{}3 {\r A} following estimation and correction of the distortion. We also use a 70S ribosome sample to demonstrate that in addition to affecting resolution, magnification distortion can also interfere with the classification of heterogeneous data.</p>
},
	keywords = {Anisotropy, Cryoelectron Microscopy, Gold, Image Enhancement},
	issn = {1095-8657},
	doi = {10.1016/j.jsb.2015.08.006},
	author = {Grant, Timothy and Grigorieff, Nikolaus}
}
