@article {47973,
	title = {Motion quantification during multi-photon functional imaging in behaving animals.},
	journal = {Biomedical Optics Express},
	volume = {7},
	year = {2016},
	month = {2016 Sep 1},
	pages = {3686-3695},
	abstract = {<p>Functional imaging in behaving animals is essential to understanding brain function. However, artifacts resulting from animal motion, including locomotion, can severely corrupt functional measurements. To dampen tissue motion, we designed a new optical window with minimal optical aberrations. Using the newly developed high-speed continuous volumetric imaging system based on an optical phase-locked ultrasound lens, we quantified motion of the cerebral cortex and hippocampal surface during two-photon functional imaging in behaving mice. We find that the out-of-plane motion is generally greater than the axial dimension of the point-spread-function during mouse locomotion, which indicates that high-speed continuous volumetric imaging is necessary to minimize motion artifacts.</p>
},
	doi = {10.1364/BOE.7.003686},
	author = {Kong, Lingjie and Little, Justin P and Cui, Meng}
}
