@article {48415,
	title = {AMPK and vacuole-associated Atg14p orchestrate {\textmu}-lipophagy for energy production and long-term survival under glucose starvation.},
	journal = {eLife},
	volume = {6},
	year = {2017},
	month = {2017 Apr 10},
	pages = {e21690},
	abstract = {<p>Dietary restriction increases the longevity of many organisms but the cell signaling and organellar mechanisms underlying this capability are unclear. We demonstrate that to permit long-term survival in response to sudden glucose depletion, yeast cells activate lipid-droplet (LD) consumption through micro-lipophagy ({\textmu}-lipophagy), in which fat is metabolized as an alternative energy source. AMP-activated protein kinase (AMPK) activation triggered this pathway, which required Atg14p. More gradual glucose starvation, amino acid deprivation or rapamycin did not trigger {\textmu}-lipophagy and failed to provide the needed substitute energy source for long-term survival. During acute glucose restriction, activated AMPK was stabilized from degradation and interacted with Atg14p. This prompted Atg14p redistribution from ER exit sites onto liquid-ordered vacuole membrane domains, initiating {\textmu}-lipophagy. Our findings that activated AMPK and Atg14p are required to orchestrate {\textmu}-lipophagy for energy production in starved cells is relevant for studies on aging and evolutionary survival strategies of different organisms.</p>
},
	issn = {2050-084X},
	doi = {10.7554/eLife.21690},
	author = {Seo, Arnold Young and Lau, Pick-Wei and Feliciano, Daniel and Sengupta, Prabuddha and Le Gros, Mark A and Cinquin, Bertrand and Larabell, Carolyn A and Lippincott-Schwartz, Jennifer}
}
