@article {48420,
	title = {Teaching old dyes new tricks: biological probes built from fluoresceins and rhodamines.},
	journal = {Annual Review of Biochemistry},
	volume = {86},
	year = {2017},
	month = {2017 Apr 07},
	pages = {825-43},
	abstract = {<p>Small-molecule fluorophores, such as fluorescein and rhodamine derivatives, are critical tools in modern biochemical and biological research. The field of chemical dyes is old; colored molecules were first discovered in the 1800s, and the fluorescein and rhodamine scaffolds have been known for over a century. Nevertheless, there has been a renaissance in using these dyes to create tools for biochemistry and biology. The application of modern chemistry, biochemistry, molecular genetics, and optical physics to these old structures enables and drives the development of novel, sophisticated fluorescent dyes. This critical review focuses on an important example of chemical biology-the melding of old and new chemical knowledge-leading to useful molecules for advanced biochemical and biological experiments. Expected final online publication date for the Annual Review of Biochemistry Volume 86 is June 20, 2017. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.</p>
},
	issn = {1545-4509},
	doi = {10.1146/annurev-biochem-061516-044839},
	author = {Lavis, Luke D}
}
