@article {48650,
	title = {Rational engineering of photoconvertible fluorescent proteins for dual-color fluorescence nanoscopy enabled by a triplet-state mechanism of primed conversion.},
	journal = {Angewandte Chemie (International ed. in English)},
	volume = {56},
	year = {2017},
	month = {2017 Jun 29},
	pages = {11628-33},
	abstract = {<p>Green-to-red photoconvertible fluorescent proteins (pcFPs) are powerful tools for super-resolution localization microscopy and protein tagging. Recently, they have been found to undergo efficient photoconversion not only by the traditional 400-nm illumination but also by an alternative method termed primed conversion, employing dual wavelength illumination with blue and far-red/near-infrared light. Primed conversion has been reported only for Dendra2 and its mechanism has remained elusive. Here, we uncover the molecular mechanism of primed conversion by reporting the intermediate "primed" state to be a triplet dark state formed by intersystem crossing. We show that formation of this state can be influenced by the introduction of serine or threonine at sequence position 69 (Eos notation) and use this knowledge to create "pr"- (for primed convertible) variants of most known green-to-red pcFPs.</p>
},
	issn = {1521-3773},
	doi = {10.1002/anie.201706121},
	author = {Mohr, Manuel Alexander and Kobitski, Andrei Yu and Sabater, Lluc Rullan and Nienhaus, Karin and Obara, Christopher John and Lippincott-Schwartz, Jennifer and Nienhaus, Gerd Ulrich and Pantazis, Periklis}
}
