@article {48801,
	title = {Labeling cellular structures in vivo using confined primed conversion of photoconvertible fluorescent proteins.},
	journal = {Nature Protocols},
	volume = {11},
	year = {2016},
	month = {2016 Dec},
	pages = {2419-2431},
	abstract = {<p>The application of green-to-red photoconvertible fluorescent proteins (PCFPs) for in vivo studies in complex 3D tissue structures has remained limited because traditional near-UV photoconversion is not confined in the axial dimension, and photomodulation using axially confined, pulsed near-IR (NIR) lasers has proven inefficient. Confined primed conversion is a dual-wavelength continuous-wave (CW) illumination method that is capable of axially confined green-to-red photoconversion. Here we present a protocol to implement this technique with a commercial confocal laser-scanning microscope (CLSM); evaluate its performance on an in vitro setup; and apply primed conversion for in vivo labeling of single cells in developing zebrafish and mouse preimplantation embryos expressing the green-to-red photoconvertible protein Dendra2. The implementation requires a basic understanding of laser-scanning microscopy, and it can be performed within a single day once the required filter cube is manufactured.</p>
},
	keywords = {Animals, Light, Luminescent Proteins, Mice, Microscopy, Confocal, Staining and Labeling, Zebrafish},
	issn = {1750-2799},
	doi = {10.1038/nprot.2016.134},
	author = {Mohr, Manuel Alexander and Argast, Paul and Pantazis, Periklis}
}
