@article {48938,
	title = {Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis.},
	journal = {Nature Communications},
	volume = {8},
	year = {2017},
	month = {2017 Nov 10},
	pages = {1412},
	abstract = {<p>The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe "semisynthetic" pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Orange that match the properties of SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization of both exocytosis and endocytosis, constituting new bright sensors for these key steps of synaptic transmission.</p>
},
	issn = {2041-1723},
	doi = {10.1038/s41467-017-01752-5},
	author = {Martineau, Magalie and Somasundaram, Agila and Grimm, Jonathan B and Gruber, Todd D and Choquet, Daniel and Taraska, Justin W and Lavis, Luke D and Perrais, David}
}
