@article {49576,
	title = {Whole-cell, 3D and multi-color STED imaging with exchangeable fluorophores.},
	journal = {Nano Letters},
	volume = {19},
	year = {2018},
	month = {2018 Dec 10},
	pages = {500-5},
	abstract = {<p>We demonstrate STED microscopy of whole bacterial and eukaryotic cells using fluorogenic labels that reversibly bind to their target structure. A constant exchange of labels guarantees the removal of photobleached fluorophores and their replacement by intact fluorophores, thereby circumventing bleaching-related limitations of STED super-resolution imaging. We achieve a constant labeling density and demonstrate a fluorescence signal for long and theoretically unlimited acquisition times. Using this concept, we demonstrate whole-cell, 3D, multi-color and live cell STED microscopy.</p>
},
	issn = {1530-6992},
	doi = {10.1021/acs.nanolett.8b04385},
	author = {Spahn, Christoph and Grimm, Jonathan B and Lavis, Luke D and Lampe, Marko and Heilemann, Mike}
}
