@article {64831,
	title = {Cytoskeletal actin patterns shape mast cell activation.},
	journal = {Communications Biology},
	volume = {2},
	year = {2019},
	month = {2019},
	pages = {93},
	abstract = {<p>Activation of immune cells relies on a dynamic actin cytoskeleton. Despite detailed knowledge of molecular actin assembly, the exact processes governing actin organization during activation remain elusive. Using advanced microscopy, we here show that Rat Basophilic Leukemia (RBL) cells, a model mast cell line, employ an orchestrated series of reorganization events within the cortical actin network during activation. In response to IgE antigen-stimulation of FCε receptors (FCεR) at the RBL cell surface, we observed symmetry breaking of the F-actin network and subsequent rapid disassembly of the actin cortex. This was followed by a reassembly process that may be driven by the coordinated transformation of distinct nanoscale F-actin architectures, reminiscent of self-organizing actin patterns. Actin patterns co-localized with zones of Arp2/3 nucleation, while network reassembly was accompanied by myosin-II activity. Strikingly, cortical actin disassembly coincided with zones of granule secretion, suggesting that cytoskeletal actin patterns contribute to orchestrate RBL cell activation.</p>
},
	issn = {2399-3642},
	doi = {10.1038/s42003-019-0322-9},
	author = {Colin-York, Huw and Li, Dong and Korobchevskaya, Kseniya and Chang, Veronica T and Betzig, Eric and Eggeling, Christian and Fritzsche, Marco}
}
