@article {65057,
	title = {Light-sheet microscopy and its potential for understanding developmental processes.},
	journal = {Annual Review of Cell and Developmental Biology},
	volume = {35},
	year = {2019},
	month = {2019 Oct 6},
	pages = {655-81},
	abstract = {<p>The ability to visualize and quantitatively measure dynamic biological processes in vivo and at high spatiotemporal resolution is of fundamental importance to experimental investigations in developmental biology. Light-sheet microscopy is particularly well suited to providing such data, since it offers exceptionally high imaging speed and good spatial resolution while minimizing light-induced damage to the specimen. We review core principles and recent advances in light-sheet microscopy, with a focus on concepts and implementations relevant for applications in developmental biology. We discuss how light-sheet microcopy has helped advance our understanding of developmental processes from single-molecule to whole-organism studies, assess the potential for synergies with other state-of-the-art technologies, and introduce methods for computational image and data analysis. Finally, we explore the future trajectory of light-sheet microscopy, discuss key efforts to disseminate new light-sheet technology, and identify exciting opportunities for further advances.</p>
},
	issn = {1530-8995},
	doi = {10.1146/annurev-cellbio-100818-125311},
	author = {Wan, Yinan and McDole, Katie and Keller, Philipp J}
}
