@article {65236,
	title = {Preparation and co-culture of iPSC-derived dopaminergic neurons and astrocytes.},
	journal = {Current Protocols in Cell Biology},
	volume = {85},
	year = {2019},
	month = {2019 Dec 04},
	pages = {e98},
	abstract = {<p>Induced pluripotent stem cell (iPSC)-based models are powerful tools to study neurodegenerative diseases such as Parkinson{\textquoteright}s disease. The differentiation of patient-derived neurons and astrocytes allows investigation of the molecular mechanisms responsible for disease onset and development. In particular, these two cell types can be mono- or co-cultured to study the influence of cell-autonomous and non-cell-autonomous contributors to neurodegenerative diseases. We developed a streamlined procedure to produce high-quality/high-purity cultures of dopaminergic neurons and astrocytes that originate from the same population of midbrain floor-plate progenitors. This unit describes differentiation, quality control, culture parameters, and troubleshooting tips to ensure the highest quality and reproducibility of research results. {\textcopyright} 2019 The Authors. Basic Protocol 1: Differentiation of iPSCs into midbrain-patterned neural progenitor cells Support Protocol: Quality control of neural progenitor cells Basic Protocol 2: Differentiation of neural progenitor cells into astrocytes Basic Protocol 3: Differentiation of neural progenitor cells into dopaminergic neurons Basic Protocol 4: Co-culture of iPSC-derived neurons and astrocytes.</p>
},
	issn = {1934-2616},
	doi = {10.1002/cpcb.98},
	author = {de Rus Jacquet, Aurelie}
}
