@article {65622,
	title = {Enhanced Golic+: Highly effective CRISPR gene targeting and transgene HACKing in .},
	journal = {Development},
	year = {2020},
	month = {2020 May 28},
	abstract = {<p>Gene targeting is an incredibly valuable technique. Sometimes however, it can also be extremely challenging for various intrinsic reasons (e.g. low target accessibility or nature/extent of gene modification). To bypass these barriers, we designed a transgene-based system in Drosophila that increases the number of independent gene targeting events while at the same time enriching for correctly targeted progeny. Unfortunately, with particularly challenging gene targeting experiments, our original design yielded numerous false positives. Here we deliver a much-improved technique named Enhanced Golic+ (E-Golic+). E-Golic+ incorporates genetic modifications to tighten lethality-based selection while simultaneously boosting efficiency. With E-Golic+, we easily achieve previously unattainable gene targeting. Additionally, we built an E-Golic+ based, high-efficiency genetic pipeline for transgene swapping. We demonstrate its utility by transforming GAL4 enhancer-trap lines into tissue-specific Cas9-expressing lines. Given the superior efficiency, specificity and scalability, E-Golic+ promises to expedite development of additional sophisticated genetic/genomic tools in .</p>
},
	issn = {1477-9129},
	doi = {10.1242/dev.181974},
	author = {Chen, Hui-Min and Yao, Xiaohao and Ren, Qingzhong and Chang, Chuan-Chie and Liu, Ling-Yu and Miyares, Rosa Linda and Lee, Tzumin}
}
