@article {65765,
	title = {A split fluorescent reporter with rapid and reversible complementation.},
	journal = {Nature communications},
	volume = {10},
	year = {2019},
	month = {06/2019},
	pages = {2822},
	abstract = {<p>Interactions between proteins play an essential role in metabolic and signaling pathways, cellular processes and organismal systems. We report the development of splitFAST, a fluorescence complementation system for the visualization of transient protein-protein interactions in living cells. Engineered from the fluorogenic reporter FAST (Fluorescence-Activating and absorption-Shifting Tag), which specifically and reversibly binds fluorogenic hydroxybenzylidene rhodanine (HBR) analogs, splitFAST displays rapid and reversible complementation, allowing the real-time visualization of both the formation and the dissociation of a protein assembly.</p>
},
	doi = {10.1038/s41467-019-10855-0},
	author = {Tebo, Alison G and Gautier, Arnaud}
}
