@article {67702,
	title = {Pervasive fold switching in a ubiquitous protein superfamily.},
	journal = {bioRxiv},
	year = {2021},
	month = {2021 Jun 11},
	abstract = {<p>Fold-switching proteins challenge the one-sequence-one-structure paradigm by adopting multiple stable folds. Nevertheless, it is uncertain whether fold switchers are naturally pervasive or rare exceptions to the well-established rule. To address this question, we developed a predictive method and applied it to the NusG superfamily of \&gt;15,000 transcription factors. We predicted that a substantial population (25\%) of the proteins in this family switch folds. Circular dichroism and nuclear magnetic resonance spectroscopies of 10 sequence-diverse variants confirmed our predictions. Thus, we leveraged family-wide predictions to determine both conserved contacts and taxonomic distributions of fold-switching proteins. Our results indicate that fold switching is pervasive in the NusG superfamily and that the single-fold paradigm significantly biases structure-prediction strategies.</p>
},
	doi = {10.1101/2021.06.10.447921},
	author = {Lauren L. Porter and Allen K. Kim and Loren L. Looger and Anaya Majumdar and Mary Starich}
}
