@article {68370,
	title = {A proliferative to invasive switch is mediated by srGAP1 downregulation through the activation of TGF-β2 signaling.},
	journal = {Cell Reports},
	volume = {40},
	year = {2022},
	month = {2022 Sep 20},
	pages = {111358},
	abstract = {<p>Many breast cancer (BC) patients suffer from complications of metastatic disease. To form metastases, cancer cells must become migratory and coordinate both invasive and proliferative programs at distant organs. Here, we identify srGAP1 as a regulator of a proliferative-to-invasive switch in BC cells. High-resolution light-sheet microscopy demonstrates that BC cells can form actin-rich protrusions during extravasation. srGAP1 cells display a motile and invasive phenotype that facilitates their extravasation from blood vessels, as shown in zebrafish and mouse models, while attenuating tumor growth. Interestingly, a population of srGAP1 cells remain as solitary disseminated tumor cells in the lungs of mice bearing BC tumors. Overall, srGAP1 cells have increased Smad2 activation and TGF-β2 secretion, resulting in increased invasion and p27 levels to sustain quiescence. These findings identify srGAP1 as a mediator of a proliferative to invasive phenotypic switch in BC cells in\&nbsp;vivo through a TGF-β2-mediated signaling axis.</p>
},
	keywords = {Actins, Animals, Cell Line, Tumor, Down-Regulation, Mice, Transforming Growth Factor beta2, Zebrafish},
	issn = {2211-1247},
	doi = {10.1016/j.celrep.2022.111358},
	author = {Mondal, Chandrani and Gacha-Garay, Majo J and Larkin, Kathryn A and Adikes, Rebecca C and Di Martino, Julie S and Chien, Chen-Chi and Fraser, Madison and Eni-Aganga, Ireti and Agullo-Pascual, Esperanza and Cialowicz, Katarzyna and Ozbek, Umut and Naba, Alexandra and Gaitas, Angelo and Fu, Tian-Ming and Upadhyayula, Srigokul and Betzig, Eric and Matus, David Q and Martin, Benjamin L and Bravo-Cordero, Jose Javier}
}
