@article {68753,
	title = {Basement Membrane Regulates Fibronectin Organization Using Sliding Focal Adhesions Driven by a Contractile Winch},
	journal = {Developmental Cell},
	volume = {52},
	year = {2020},
	month = {03/2020},
	pages = {631-646.e4},
	abstract = {<p>Summary We have discovered that basement membrane and its major components can induce rapid, strikingly robust fibronectin organization. In this new matrix assembly mechanism, α5β1 integrin-based focal adhesions slide actively on the underlying matrix toward the ventral cell center through the dynamic shortening of myosin IIA-associated actin stress fibers to drive rapid fibronectin fibrillogenesis distal to the adhesion. This mechanism contrasts with classical fibronectin assembly based on stable or fixed-position focal adhesions containing αVβ3 integrins plus α5β1 integrin translocation into proximal fibrillar adhesions. On basement membrane components, these sliding focal adhesions contain standard focal adhesion constituents but completely lack classical αVβ3 integrins. Instead, peripheral α3β1 or α2β1 adhesions mediate initial cell attachment but over time are switched to α5β1 integrin-based sliding focal adhesions to assemble fibronectin matrix. This basement-membrane-triggered mechanism produces rapid fibronectin fibrillogenesis, providing a mechanistic explanation for the well-known widespread accumulation of fibronectin at many organ basement membranes.</p>
},
	keywords = {basement membrane, Extracellular Matrix, fibrillogenesis, fibronectin, focal adhesion, integrin, matrix assembly},
	issn = {1534-5807},
	doi = {https://doi.org/10.1016/j.devcel.2020.01.007},
	url = {https://www.sciencedirect.com/science/article/pii/S1534580720300083},
	author = {Jiaoyang Lu and Andrew D. Doyle and Yoshinari Shinsato and Shaohe Wang and Molly A. Bodendorfer and Minhua Zheng and Kenneth M. Yamada}
}
