@article {68767,
	title = {The Microtubule Minus-End-Binding Protein Patronin/PTRN-1 Is Required for Axon Regeneration in C.~elegans},
	journal = {Cell Reports},
	volume = {9},
	year = {2014},
	month = {11/2014},
	pages = {874-883},
	abstract = {<p>Summary Precise regulation of microtubule (MT) dynamics is increasingly recognized as a critical determinant of\&nbsp;axon regeneration. In contrast to developing neurons, mature axons exhibit noncentrosomal microtubule nucleation. The factors regulating noncentrosomal MT architecture in axon regeneration remain poorly understood. We report that PTRN-1, the C.\&nbsp;elegans member of the Patronin/Nezha/calmodulin- and spectrin-associated protein (CAMSAP) family of microtubule minus-end-binding proteins, is critical for efficient axon regeneration in\&nbsp;vivo. ptrn-1-null mutants display generally normal developmental axon outgrowth but significantly impaired regenerative regrowth after laser axotomy. Unexpectedly, mature axons in ptrn-1 mutants display elevated numbers of dynamic axonal MTs before and after injury, suggesting that PTRN-1 inhibits MT dynamics. The CKK domain of PTRN-1 is necessary and sufficient for its functions in axon regeneration and MT dynamics and appears to stabilize MTs independent of minus-end localization. Whereas in developing neurons, PTRN-1 inhibits activity of the DLK-1 mitogen-activated protein kinase (MAPK) cascade, we find that, in regeneration, PTRN-1 and DLK-1 function together to promote axonal regrowth.</p>
},
	issn = {2211-1247},
	doi = {https://doi.org/10.1016/j.celrep.2014.09.054},
	url = {https://www.sciencedirect.com/science/article/pii/S2211124714008377},
	author = {Marian Chuang and Alexandr Goncharov and Shaohe Wang and Karen Oegema and Yishi Jin and Andrew~D. Chisholm}
}
