@article {68791,
	title = {Mapping memories: pulse-chase labeling reveals AMPA receptor dynamics during memory formation.},
	journal = {bioRxiv},
	year = {2023},
	month = {2023 May 29},
	abstract = {<p>A tool to map changes in synaptic strength during a defined time window could provide powerful insights into the mechanisms governing learning and memory. We developed a technique, Extracellular Protein Surface Labeling in Neurons (EPSILON), to map α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) insertion\&nbsp;<em>in vivo</em>\&nbsp;by pulse-chase labeling of surface AMPARs with membrane-impermeable dyes. This approach allows for single-synapse resolution maps of plasticity in genetically targeted neurons during memory formation. We investigated the relationship between synapse-level and cell-level memory encodings by mapping synaptic plasticity and cFos expression in hippocampal CA1 pyramidal cells upon contextual fear conditioning (CFC). We observed a strong correlation between synaptic plasticity and cFos expression, suggesting a synaptic mechanism for the association of cFos expression with memory engrams. The EPSILON technique is a useful tool for mapping synaptic plasticity and may be extended to investigate trafficking of other transmembrane proteins.</p>
},
	doi = {10.1101/2023.05.26.541296},
	url = {https://doi.org/10.1101/2023.05.26.541296},
	author = {Doyeon Kim and Pojeong Park and Xiuyuan Li and J. David Wong Campos and He Tian and Eric M. Moult and Jonathan B. Grimm and Luke Lavis and Adam E. Cohen}
}
