@article {69110, title = {Imaging neuronal voltage beyond the scattering limit}, journal = {bioRxiv}, year = {2023}, month = {2023 Dec 05}, abstract = {

Voltage imaging is a promising technique for high-speed recording of neuronal population activity. However, tissue scattering severely limits its application in dense neuronal populations. Here, we adopted the principle of localization microscopy, a technique that enables super-resolution imaging of single-molecules, to resolve dense neuronal activities\ in vivo. Leveraging the sparse activation of neurons during action potentials (APs), we precisely localize the fluorescence change associated with each AP, creating a super-resolution image of neuronal activities. This approach, termed\ Activity\ Localization\ Imaging (ALI), identifies overlapping neurons and separates their activities with over 10-fold greater precision than what tissue scattering permits. Using ALI, we simultaneously recorded over a hundred densely-labeled CA1 neurons, creating a map of hippocampal theta oscillation at single-cell and single-cycle resolution.

}, doi = {10.1101/2023.12.03.568403}, url = {https://doi.org/10.1101/2023.12.03.568403}, author = {Tsai-Wen Chen and Xian-Bin Huang and Sarah E. Plutkis and Katie L. Holland and Luke D. Lavis and Bei-Jung Lin} }