@article {69136,
	title = {Permanent deconstruction of intracellular primary cilia in differentiating granule cell neurons.},
	journal = {J Cell Biol.},
	volume = {223},
	year = {2024},
	month = {2024 Aug 13},
	pages = {e202404038},
	abstract = {<p>Primary cilia on granule cell neuron progenitors in the developing cerebellum detect sonic hedgehog to facilitate proliferation. Following differentiation, cerebellar granule cells become the most abundant neuronal cell type in the brain. While granule cell cilia are essential during early developmental stages, they become infrequent upon maturation. Here, we provide nanoscopic resolution of cilia in situ using large-scale electron microscopy volumes and immunostaining of mouse cerebella. In many granule cells, we found intracellular cilia, concealed from the external environment. Cilia were disassembled in differentiating granule cell neurons-in a process we call cilia deconstruction-distinct from premitotic cilia resorption in proliferating progenitors. In differentiating granule cells, cilia deconstruction involved unique disassembly intermediates, and, as maturation progressed, mother centriolar docking at the plasma membrane. Unlike ciliated neurons in other brain regions, our results show the deconstruction of concealed cilia in differentiating granule cells, which might prevent mitogenic hedgehog responsiveness. Ciliary deconstruction could be paradigmatic of cilia removal during differentiation in other tissues.</p>
},
	doi = {10.1016/j.cub.2024.07.036},
	url = {https://doi.org/10.1016/j.cub.2024.07.036 },
	author = {Ott, Carolyn M and Constable, Sandii and Nguyen, Tri M and White, Kevin and Lee, Wei-Chung Allen and Lippincott-Schwartz, Jennifer and Mukhopadhyay, Saikat}
}
