@article {69403,
	title = {Connectomic Analysis of Mitochondria in the Central Brain of Drosophila},
	journal = {bioRxiv},
	year = {2024},
	month = {2024 Apr 25},
	abstract = {<p>Mitochondria are an integral part of the metabolism of a neuron. EM images of fly brain volumes, taken for connectomics, contain mitochondria as well as the cells and synapses that have already been reported. Here, from the Drosophila hemibrain dataset, we extract, classify, and measure approximately 6 million mitochondria among roughly 21 thousand neurons of more than 5500 cell types. Each mitochondrion is classified by its appearance - dark and dense, light and sparse, or intermediate - and the location, orientation, and size (in voxels) are annotated. These mitochondria are added to our publicly available data portal, and each synapse is linked to its closest mitochondrion. Using this data, we show quantitative evidence that mitochodrial trafficing extends to the smallest dimensions in neurons. The most basic characteristics of mitochondria - volume, distance from synapses, and color - vary considerably between cell types, and between neurons with different neurotransmitters. We find that polyadic synapses with more post-synaptic densities (PSDs) have closer and larger mitochondria on the pre-synaptic side, but smaller and more distant mitochondria on the PSD side. We note that this relationship breaks down for synapses with only one PSD, suggesting a different role for such synapses.Competing Interest StatementThe authors have declared no competing interest.</p>
},
	doi = {10.1101/2024.04.21.590464},
	url = {https://www.biorxiv.org/content/early/2024/04/25/2024.04.21.590464},
	author = {Patricia K Rivlin and Michal Januszewski and Kit D Longden and Erika Neace and Louis K Scheffer and Christopher Ordish and Jody Clements and Elliott Phillips and Natalie Smith and Satoko Takemura and Lowell Umayam and Claire Walsh and Emily A Yakal and Stephen M Plaza and Stuart Berg}
}
