@article {69424,
	title = {Adaptive optical third-harmonic generation microscopy for in vivo imaging of tissues},
	journal = {bioRxiv},
	year = {2024},
	month = {2024 May 05},
	abstract = {<p>Third-harmonic generation microscopy is a powerful label-free nonlinear imaging technique, providing essential information about structural characteristics of cells and tissues without requiring external labelling agents. In this work, we integrated a recently developed compact adaptive optics module into a third-harmonic generation microscope, to measure and correct for optical aberrations in complex tissues. Taking advantage of the high sensitivity of the third-harmonic generation process to material interfaces and thin membranes, along with the 1,300-nm excitation wavelength used here, our adaptive optical third-harmonic generation microscope enabled high-resolution in vivo imaging within highly scattering biological model systems. Examples include imaging of myelinated axons and vascular structures within the mouse spinal cord and deep cortical layers of the mouse brain, along with imaging of key anatomical features in the roots of the model plant Brachypodium distachyon. In all instances, aberration correction led to significant enhancements in image quality.</p>
},
	doi = {10.1101/2024.05.02.592275},
	url = {https://www.biorxiv.org/content/early/2024/05/05/2024.05.02.592275},
	author = {Cristina Rodr{\'\i}guez and Daisong Pan and Ryan G. Natan and Manuel A. Mohr and Max Miao and Xiaoke Chen and Trent R. Northen and John P. Vogel and Na Ji}
}
