@article {69443,
	title = {Spatial mapping of hepatic ER and mitochondria architecture reveals zonated remodeling in fasting and obesity},
	journal = {Nat Commun},
	volume = {15},
	year = {2024},
	month = {2024 May 10},
	pages = {3982},
	abstract = {<p>The hepatocytes within the liver present an immense capacity to adapt to changes in nutrient availability. Here, by using high resolution volume electron microscopy, we map how hepatic subcellular spatial organization is regulated during nutritional fluctuations and as a function of liver zonation. We identify that fasting leads to remodeling of endoplasmic reticulum (ER) architecture in hepatocytes, characterized by the induction of single rough ER sheet around the mitochondria, which becomes larger and flatter. These alterations are enriched in periportal and mid-lobular hepatocytes but not in pericentral hepatocytes. Gain- and loss-of-function in vivo models demonstrate that the Ribosome receptor binding protein1 (RRBP1) is required to enable fasting-induced ER sheet-mitochondria interactions and to regulate hepatic fatty acid oxidation. Endogenous RRBP1 is enriched around periportal and mid-lobular regions of the liver. In obesity, ER-mitochondria interactions are distinct and fasting fails to induce rough ER sheet-mitochondrion interactions. These findings illustrate the importance of a regulated molecular architecture for hepatocyte metabolic flexibility.</p>
},
	keywords = {Animals, Endoplasmic Reticulum, Fasting, Fatty Acids, Hepatocytes, Humans, Liver, Male, Mice, Mice, Inbred C57BL, Mitochondria, Mitochondria, Liver, Obesity, Oxidation-Reduction, Ribosomal Proteins},
	issn = {2041-1723},
	doi = {10.1038/s41467-024-48272-7},
	author = {Parlakg{\"u}l, G{\"u}ne{\c s} and Pang, Song and Artico, Leonardo L and Min, Nina and Cagampan, Erika and Villa, Reyna and Goncalves, Renata L S and Lee, Grace Yankun and Xu, C Shan and Hotam{\i}{\c s}l{\i}gil, G{\"o}khan S and Arruda, Ana Paula}
}
