@article {70048,
	title = {Cryo-electron Microscopy of Vitreous Sections (CEMOVIS) Application for Model Organisms},
	journal = {Microscopy and Microanalysis},
	volume = {31},
	year = {2025},
	month = {2025 Jul 25},
	pages = {ozaf048.484},
	abstract = {<p>Cryo-ultramicrotomy, developed by Bernhard in 1965 [1], has long been regarded as the pinnacle of achievement for electron microscopists. This technique allows biological samples to be sliced into ultrathin sections and examined in a cryo-electron microscope, revealing the most intricate subcellular structures without chemical fixation or staining. The advent of vitrification [2,3] and high-pressure freezing (HPF) technology [4,5] provided reliable methods for preserving cellular structures, and the introduction of diamond knife to cryo-ultramicrotomy [6] offering cryo-ultramicrotomists reassurance in consistency of the quality [7].</p>
},
	issn = {1431-9276},
	doi = {10.1093/mam/ozaf048.484},
	url = {https://doi.org/10.1093/mam/ozaf048.484},
	author = {Uryu, Kunihiro and Zhao, Xiaowei and Shiozaki, Momoko and Yu, Zhiheng}
}
