@article {70359,
	title = {Adapting upright light sheet fluorescence microscopy for imaging at air-liquid interface},
	journal = {bioRxiv},
	year = {2026},
	month = {2026 Apr 09},
	abstract = {<p>Light sheet fluorescence microscopy (LSFM) is increasingly appreciated as the gold standard for gentle, volumetric imaging with fast acquisition speeds and/or long imaging durations. However, the often-constrained sample space of these microscopes has precluded a specific class of biological specimens from being studied with these tools: those requiring an air-liquid interface (ALI). Here, we present a device for robust imaging at ALI on an upright light sheet microscope with dipping objectives. We demonstrate the system using three relevant use-cases: ex vivo embryonic mouse salivary glands, human epidermal equivalent cultures, and in vivo adult Drosophila melanogaster brains. While the device presented is engineered for one specific light sheet microscope design, it provides a blueprint for easy adaptation to other systems. In doing so, it can potentially spur the use of LSFM for model systems that have so far been unable to take advantage of this powerful technology.</p>
},
	doi = {10.64898/2026.04.07.716945},
	url = {https://www.biorxiv.org/content/early/2026/04/09/2026.04.07.716945},
	author = {Hobson, Chad M. and Izumi, Kenji and Aaron, Jesse S. and Bharathan, Navaneetha Krishnan and Ceriani, M. Fernanda and Giang, William and Ispizua, Juan I. and Kowalczyk, Andrew P. and Lee, Rachel M. and Morales, E. Angelo and Puls, Owen F. and Quarles, Ellen and Rodriguez-Caron, Micaela and Stahley, Sara N. and Tassara, Francisco and Wang, Shaohe and Yao, Shunze and Tsuchiya, Takanori and Chew, Teng-Leong}
}
