@article {70485,
	title = {A Yeast Two-Hybrid Assay to Develop Optogenetic Tools for Controlling Protein-Protein Interactions.},
	journal = {Methods Mol Biol},
	volume = {3032},
	year = {2026},
	month = {2026 Aug 02},
	pages = {69-83},
	abstract = {<p>Yeast two hybrid (Y2H) assays serve as powerful platforms for the detection and engineering of protein-protein interactions. Here, we describe a strategy for using Y2H-based screening to assess and develop optogenetic tools based on light-controlled protein-protein interactions.</p>
},
	keywords = {High-Throughput Screening Assays, Optogenetics, Protein Binding, Protein Engineering, Protein Interaction Mapping, Saccharomyces cerevisiae, Two-Hybrid System Techniques},
	issn = {1940-6029},
	doi = {10.1007/978-1-0716-5257-2_5},
	url = {https://doi.org/10.1007/978-1-0716-5257-2_5},
	author = {Woloschuk, Ryan M and Woolley, G Andrew}
}
