@article {67234,
	title = {High-precision coding in visual cortex.},
	journal = {Cell},
	volume = {184},
	year = {2021},
	month = {2021 May 13},
	pages = {2767-78},
	abstract = {<p>Individual neurons in visual cortex provide the brain with unreliable estimates of visual features. It is not known whether the single-neuron variability is correlated across large neural populations, thus impairing the global encoding of stimuli. We recorded simultaneously from up to 50,000 neurons in mouse primary visual cortex (V1) and in higher order visual areas and measured stimulus discrimination thresholds of 0.35{\textdegree} and 0.37{\textdegree}, respectively, in an orientation decoding task. These neural thresholds were almost 100 times smaller than the behavioral discrimination thresholds reported in mice. This discrepancy could not be explained by stimulus properties or arousal states. Furthermore, behavioral variability during a sensory discrimination task could not be explained by neural variability in V1. Instead, behavior-related neural activity arose dynamically across a network of non-sensory brain areas. These results imply that perceptual discrimination in mice is limited by downstream decoders, not by neural noise in sensory representations.</p>
},
	issn = {1097-4172},
	doi = {10.1016/j.cell.2021.03.042},
	author = {Stringer, Carsen and Michaelos, Michalis and Tsyboulski, Dmitri and Lindo, Sarah E and Pachitariu, Marius}
}
@article {65241,
	title = {Dual-plane 3-photon microscopy with remote focusing.},
	journal = {Biomedical Optics Express},
	volume = {10},
	year = {2019},
	month = {2019 Nov 1},
	pages = {5585-5599},
	abstract = {<p>3-photon excitation enables fluorescence microscopy deep in densely labeled and highly scattering samples. To date, 3-photon excitation has been restricted to scanning a single focus, limiting the speed of volume acquisition. Here, for the first time to our knowledge, we implemented and characterized dual-plane 3-photon microscopy with temporal multiplexing and remote focusing, and performed simultaneous calcium imaging of two planes beyond 600 {\textmu}m deep in the cortex of a pan-excitatory GCaMP6s transgenic mouse with a per-plane framerate of 7 Hz and an effective 2 MHz laser repetition rate. This method is a straightforward and generalizable modification to single-focus 3PE systems, doubling the rate of volume (column) imaging with off-the-shelf components and minimal technical constraints.</p>
},
	issn = {2156-7085},
	doi = {10.1364/BOE.10.005585},
	author = {Takasaki, Kevin T and Tsyboulski, Dmitri and Waters, Jack}
}
