@article {46890, title = {Continuous volumetric imaging via an optical phase-locked ultrasound lens.}, journal = {Nature Methods}, volume = {12}, year = {2015}, month = {2015-Jul 13}, pages = {759-62}, abstract = {

In vivo imaging at high spatiotemporal resolution is key to the understanding of complex biological systems. We integrated an optical phase-locked ultrasound lens into a two-photon fluorescence microscope and achieved microsecond-scale axial scanning, thus enabling volumetric imaging at tens of hertz. We applied this system to multicolor volumetric imaging of processes sensitive to motion artifacts, including calcium dynamics in behaving mouse brain and transient morphology changes and trafficking of immune cells.

}, doi = {10.1038/nmeth.3476}, author = {Kong, Lingjie and Tang, Jianyong and Little, Justin P and Yu, Yang and L{\"a}mmermann, Tim and Lin, Charles P and Germain, Ronald N and Cui, Meng} } @article {5102, title = {Complex wavefront corrections for deep tissue focusing using low coherence backscattered light.}, journal = {Optics Express}, volume = {20}, year = {2012}, month = {2012}, pages = {16532-43}, abstract = {

Aberrations and random scattering severely limit optical imaging in deep tissue. Adaptive optics can in principle drastically extend the penetration depth and improve the image quality. However, for random scattering media a large number of spatial modes need to be measured and controlled to restore a diffraction limited focus. Here, we present a parallel wavefront optimization method using backscattered light as a feedback. Spatial confinement of the feedback signal is realized with a confocal pinhole and coherence gating. We show in simulations and experiments that this approach enables focusing deep into tissue over up to six mean scattering path lengths. Experimentally the technique was tested on tissue phantoms and fixed brain slices.

}, doi = {10.1364/OE.20.016532}, author = {Fiolka, Reto and Si, Ke and Cui, Meng} }