@article {69903,
	title = {Challenges of microscopy technology dissemination to resource-constrained communities.},
	journal = {Nat Methods},
	year = {2025},
	month = {2025 May 06},
	abstract = {<p>No abstract available.</p>
},
	issn = {1548-7105},
	doi = {10.1038/s41592-025-02690-7},
	url = {https://doi.org/10.1038/s41592-025-02690-7},
	author = {Aaron, Jesse S and Jacobs, Caron A and Malacrida, Leonel and Keppler, Antje and French, Paul and Fletcher, Daniel A and Wood, Christopher and Brown, Claire M and Wright, Graham D and Ogawa, Satoshi and Maina, Mahmoud and Chew, Teng-Leong}
}
@article {67670,
	title = {Imaging Africa: a strategic approach to optical microscopy training in Africa.},
	journal = {Nature Methods},
	volume = {18},
	year = {2021},
	month = {2021 Aug 20},
	pages = {847-855},
	issn = {1548-7105},
	doi = {10.1038/s41592-021-01227-y},
	author = {Reiche, Michael A and Warner, Digby F and Aaron, Jesse and Khuon, Satya and Fletcher, Daniel A and Hahn, Klaus and Rogers, Kelly L and Mhlanga, Musa and Koch, Anastasia and Quaye, Wendye and Chew, Teng-Leong}
}
@article {65614,
	title = {Chloroplast Sec14-like 1 (CPSFL1) is essential for normal chloroplast development and affects carotenoid accumulation in Chlamydomonas.},
	journal = {Proceedings of the National Academy of Sciences of the U S A},
	volume = {117},
	year = {2020},
	month = {2020 Jun 2},
	pages = {1-12},
	abstract = {<p>Plastid isoprenoid-derived carotenoids serve essential roles in chloroplast development and photosynthesis. Although nearly all enzymes that participate in the biosynthesis of carotenoids in plants have been identified, the complement of auxiliary proteins that regulate synthesis, transport, sequestration, and degradation of these molecules and their isoprenoid precursors have not been fully described. To identify such proteins that are necessary for the optimal functioning of oxygenic photosynthesis, we screened a large collection of nonphotosynthetic (acetate-requiring) DNA insertional mutants of and isolated The mutant is extremely light-sensitive and susceptible to photoinhibition and photobleaching. The gene encodes a CRAL-TRIO hydrophobic ligand-binding (Sec14) domain protein. Proteins containing this domain are limited to eukaryotes, but some may have been retargeted to function in organelles of endosymbiotic origin. The mutant showed decreased accumulation of plastidial isoprenoid-derived pigments, especially carotenoids, and whole-cell focused ion-beam scanning-electron microscopy revealed a deficiency of carotenoid-rich chloroplast structures (e.g., eyespot and plastoglobules). The low carotenoid content resulted from impaired biosynthesis at a step prior to phytoene, the committed precursor to carotenoids. The CPSFL1 protein bound phytoene and β-carotene when expressed in and phosphatidic acid in vitro. We suggest that CPSFL1 is involved in the regulation of phytoene synthesis and carotenoid transport and thereby modulates carotenoid accumulation in the chloroplast.</p>
},
	issn = {1091-6490},
	doi = {10.1073/pnas.1916948117},
	author = {Garc{\'\i}a-Cerd{\'a}n, Jos{\'e} G and Schmid, Eva M and Takeuchi, Tomomi and McRae, Ian and McDonald, Kent L and Yordduangjun, Nichakarn and Hassan, Ahmed M and Grob, Patricia and Xu, C Shan and Hess, Harald F and Fletcher, Daniel A and Nogales, Eva and Niyogi, Krishna K}
}
