@article {67753,
	title = {An open-access volume electron microscopy atlas of whole cells and tissues.},
	journal = {Nature},
	volume = {599},
	year = {2021},
	month = {2021 Nov 1},
	pages = {147-51},
	abstract = {<p>Understanding cellular architecture is essential for understanding biology. Electron microscopy (EM) uniquely visualizes cellular structures with nanometre resolution. However, traditional methods, such as thin-section EM or EM tomography, have limitations in that they visualize only a single slice or a relatively small volume of the cell, respectively. Focused ion beam-scanning electron microscopy (FIB-SEM) has demonstrated the ability to image small volumes of cellular samples with 4-nm isotropic voxels. Owing to advances in the\&nbsp;precision and stability of FIB milling, together with enhanced signal detection and faster SEM scanning, we have increased the volume that can be imaged with 4-nm\&nbsp;voxels by two orders of magnitude. Here we present a volume EM atlas at such resolution comprising ten three-dimensional datasets for whole cells and tissues, including cancer cells, immune cells, mouse pancreatic islets and Drosophila neural tissues. These open access data (via OpenOrganelle) represent the foundation of a field of high-resolution whole-cell volume EM and subsequent analyses, and we invite researchers to explore this atlas and\&nbsp;pose questions.</p>
},
	issn = {1476-4687},
	doi = {10.1038/s41586-021-03992-4},
	author = {Xu, C Shan and Pang, Song and Shtengel, Gleb and M{\"u}ller, Andreas and Ritter, Alex T and Hoffman, Huxley K and Takemura, Shin-ya and Lu, Zhiyuan and Pasolli, H Amalia and Iyer, Nirmala and Chung, Jeeyun and Bennett, Davis and Weigel, Aubrey V and Freeman, Melanie and Van Engelenburg, Schuyler B and Walther, Tobias C and Farese, Robert V and Lippincott-Schwartz, Jennifer and Mellman, Ira and Solimena, Michele and Hess, Harald F}
}
@article {67668,
	title = {Optimization of Fluorescent Proteins and Techniques for In-resin Correlative Light and Electron Microscopy},
	journal = {Microscopy and Microanalysis},
	volume = {26},
	year = {2020},
	month = {07/2020},
	pages = {1036{\textendash}1039},
	abstract = {<p>Correlative light and electron microscopy (CLEM) combines the power of electron microscopy, with its excellent resolution and contrast, with that of fluorescence imaging, which allows the staining of specific molecules, organelles, and cell populations. Fluorescence imaging is also readily compatible with live cells and behaving animals, facilitating real-time visualization of cellular processes, potentially followed by electron microscopic reconstruction. Super-resolution single-molecule localization microscopy is a relatively new modality that harnesses the ability of some fluorophores to photoconvert, through which localization precision better than Abbe{\textquoteright}s diffraction limit is achieved through iterative high-resolution localization of single-molecule emitters. Here we describe our lab{\textquoteright}s recent progress in the development of reagents and techniques for super-resolution single-molecule localization CLEM and their applications to biological problems.</p>
},
	doi = {10.1017/S143192762001675X},
	author = {Paez-Segala, Maria and Wang, Yalin and Iyer, Nirmala and Li, Wei-Ping and Rivlin, Patricia and Looger, Loren}
}
