@article {65632,
	title = {Two-parameter mobility assessments discriminate diverse regulatory factor behaviors in chromatin.},
	journal = {Molecular Cell},
	volume = {79},
	year = {2020},
	month = {2020 Aug 1},
	pages = {677},
	abstract = {<p>Enzymatic probes of chromatin structure reveal accessible versus inaccessible chromatin states, while super-resolution microscopy reveals a continuum of chromatin compaction states. Characterizing histone H2B movements by single-molecule tracking (SMT), we resolved chromatin domains ranging from low to high mobility and displaying different subnuclear localizations patterns. Heterochromatin constituents correlated with the lowest mobility chromatin, whereas transcription factors varied widely with regard to their respective mobility with low- or high-mobility chromatin. Pioneer transcription factors, which bind nucleosomes, can access the low-mobility chromatin domains, whereas weak or non-nucleosome binding factors are excluded from the domains and enriched in higher mobility domains. Nonspecific DNA and nucleosome binding accounted for most of the low mobility of strong nucleosome interactor FOXA1. Our analysis shows how the parameters of the mobility of chromatin-bound factors, but not their diffusion behaviors or SMT-residence times within chromatin, distinguish functional characteristics of different chromatin-interacting proteins.</p>
},
	issn = {1097-4164},
	doi = {10.1016/j.molcel.2020.05.036},
	author = {Lerner, Jonathan and Gomez-Garcia, Pablo Aurelio and McCarthy, Ryan L and Liu, Zhe and Lakadamyali, Melike and Zaret, Kenneth S}
}
@article {65903,
	title = {Two-parameter single-molecule analysis for measurement of chromatin mobility.},
	journal = {STAR Protocols},
	volume = {1},
	year = {2020},
	month = {2020 Dec 13},
	pages = {100223},
	abstract = {<p>This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to high mobility. When the analysis is applied to Halo-tagged, chromatin-binding proteins, it associates chromatin interaction properties with known functions in a way that previously used SMT parameters did not. For complete information on the use and execution of this protocol, please refer to Lerner et\&nbsp;al. (2020).</p>
},
	issn = {2666-1667},
	doi = {10.1016/j.xpro.2020.100223},
	author = {Lerner, Jonathan and Gomez-Garcia, Pablo Aurelio and McCarthy, Ryan L and Liu, Zhe and Lakadamyali, Melike and Zaret, Kenneth S}
}
