@article {65772,
	title = {Circularly Permuted Fluorogenic Proteins for the Design of Modular Biosensors.},
	journal = {ACS Chemical Biology},
	volume = {13},
	year = {2018},
	month = {09/2018},
	pages = {2392 {\textendash} 2397},
	abstract = {<p>Fluorescent reporters are essential components for the design of optical biosensors that are able to image intracellular analytes in living cells. Herein, we describe the development of circularly permuted variants of Fluorescence-Activating and absorption-Shifting Tag (FAST) and demonstrate their potential as reporting module in biosensors. Circularly permutated FAST (cpFAST) variants allow one to condition the binding and activation of a fluorogenic ligand (and thus fluorescence) to analyte recognition by coupling them with analyte-binding domains. We demonstrated their use for biosensor design by generating multicolor plug-and-play fluorogenic biosensors for imaging the intracellular levels of Ca2+ in living mammalian cells in real time.</p>
},
	doi = {10.1021/acschembio.8b00417},
	author = {Tebo, Alison G and Pimenta, Frederico M and Zoumpoulaki, Martha and Kikuti, Carlos and Sirkia, Helena and Plamont, Marie-Aude and Houdusse, Anne and Gautier, Arnaud}
}
@article {65769,
	title = {Improved Chemical-Genetic Fluorescent Markers for Live Cell Microscopy},
	journal = {Biochemistry},
	volume = {57},
	year = {2018},
	month = {11/2018},
	pages = {5648 {\textendash} 5653},
	abstract = {<p>Inducible chemical-genetic fluorescent markers are promising tools for live cell imaging requiring high spatiotemporal resolution and low background fluorescence. The fluorescence-activating and absorption shifting tag (FAST) was recently developed to form fluorescent molecular complexes with a family of small, synthetic fluorogenic chromophores (so-called fluorogens). Here, we use rational design to modify the binding pocket of the protein and screen for improved fluorescence performances with four different fluorogens. The introduction of a single mutation results in improvements in both quantum yield and dissociation constant with nearly all fluorogens tested. Our improved FAST (iFAST) allowed the generation of a tandem iFAST (td-iFAST) that forms green and red fluorescent reporters 1.6-fold and 2-fold brighter than EGFP and mCherry, respectively, while having a comparable size.</p>
},
	doi = {10.1021/acs.biochem.8b00649},
	url = {https://pubs-acs-org.accesdistant.sorbonne-universite.fr/doi/pdf/10.1021/acs.biochem.8b00649},
	author = {Tebo, Alison G and Pimenta, Frederico M and Zhang, Yu and Gautier, Arnaud}
}
