@article {4284,
	title = {Two-photon single-cell optogenetic control of neuronal activity by sculpted light.},
	journal = {Proceedings of the National Academy of Sciences of the United States of America},
	volume = {107},
	year = {2010},
	month = {2010 Jun 29},
	pages = {11981-6},
	abstract = {<p>Recent advances in optogenetic techniques have generated new tools for controlling neuronal activity, with a wide range of neuroscience applications. The most commonly used approach has been the optical activation of the light-gated ion channel channelrhodopsin-2 (ChR2). However, targeted single-cell-level optogenetic activation with temporal precessions comparable to the spike timing remained challenging. Here we report fast (\&lt; or = 1 ms), selective, and targeted control of neuronal activity with single-cell resolution in hippocampal slices. Using temporally focused laser pulses (TEFO) for which the axial beam profile can be controlled independently of its lateral distribution, large numbers of channels on individual neurons can be excited simultaneously, leading to strong (up to 15 mV) and fast (\&lt; or = 1 ms) depolarizations. Furthermore, we demonstrated selective activation of cellular compartments, such as dendrites and large presynaptic terminals, at depths up to 150 microm. The demonstrated spatiotemporal resolution and the selectivity provided by TEFO allow manipulation of neuronal activity, with a large number of applications in studies of neuronal microcircuit function in vitro and in vivo.</p>
},
	keywords = {Action Potentials, Animals, Cell Line, Dendrites, Electrophysiological Phenomena, Hippocampus, Humans, Lasers, Mice, Nerve Tissue Proteins, Neurons, Optical Phenomena, Patch-Clamp Techniques, Photic Stimulation, Photons, Presynaptic Terminals, Rats, Recombinant Fusion Proteins, Rhodopsin, Sprague-Dawley, Transgenic},
	issn = {1091-6490},
	doi = {10.1073/pnas.1006620107},
	author = {Andrasfalvy, Bertalan K and Zemelman, Boris V and Tang, Jianyong and Vaziri, Alipasha}
}
@article {5357,
	title = {Three dimensional super resolution fluorescence imaging of single bacterial cells by stereo photoactivated localization microscopy.},
	journal = {Microscopy and Microanalysis},
	volume = {15},
	year = {2009},
	month = {2009 July 26},
	pages = {564-5},
	doi = {10.1017/S1431927609095737},
	author = {Tang, Jianyong and Vaziri, Alipasha and Akerboom, Jasper P. and Looger, Loren and Shank, Charles V}
}
@article {5363,
	title = {Three-dimensional nanoscopy of biological samples.},
	journal = {2009 Conference on Lasers and Electro-Optics and Quantum Electronics and Laser Science Conference (CLEO/QELS 2009), Vols. 1-5},
	volume = {1-5},
	year = {2009},
	month = {2009 Jun 5},
	pages = {147-8},
	abstract = {<p>We have demonstrated super-resolution imaging of protein distributions in cells at depth at multiple layers with a lateral localization precision better than 50 nm. The approach is based on combining photoactivated localization microscopy with temporal focusing.</p>
},
	author = {Vaziri, Alipasha and Tang, Jianyong and Shroff, Hari and Shank, Charles V}
}
